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Qu'est-ce (qui) est DAPI staining - définition
CHEMICAL COMPOUND
4',6-diamidino-2-phenylindole; C16H15N5; DAPI staining; DAPI stain
![Endothelial cells stained with DAPI (blue), [[phalloidin]] (red) and through [[immunofluorescence]] via an [[antibody]] bound to [[fluorescein isothiocyanate]] (FITC) (green).](https://commons.wikimedia.org/wiki/Special:FilePath/FluorescentCells.jpg?width=200 "Endothelial cells stained with DAPI (blue), [[phalloidin]] (red) and through [[immunofluorescence]] via an [[antibody]] bound to [[fluorescein isothiocyanate]] (FITC) (green).")
Staining
![hematoxylin and eosin (H&E)]].](https://commons.wikimedia.org/wiki/Special:FilePath/Eosinophilic, basophilic, chromophobic and amphophilic staining.png?width=200 "hematoxylin and eosin (H&E)]].")
![Example of [[negative staining]]](https://commons.wikimedia.org/wiki/Special:FilePath/HexLamMic phases.jpg?width=200 "Example of [[negative staining]]")
TECHNIQUE USED TO ENHANCE CONTRAST OF SPECIMENS OBSERVED UNDER A MICROSCOPE
In vitro staining; Vital stains; In vivo staining; Histological staining; Histologic staining; Biological stain; Biological stains; Stain (biology); Staining (microscopy); Cell staining; Staining and labeling; Simple stain; Stain (microbiology); Staining (biology); Stain (microscopy); Staining dye; Staining dyes; Vital dye; Stain (histology); Amphophile; Amphophilic; Cell stain; Tingibility; Histologic stain; Microscopy stain; Tissue stain; Tissue staining; Histological stain; Hematological stain; Positive staining; Positive stains; Positive stain
·p.pr. & ·vb.n. of Stain.
Gram stain
![µm]] in diameter.](https://commons.wikimedia.org/wiki/Special:FilePath/Candida Gram stain.jpg?width=200 "µm]] in diameter.")
![Gram-stain of [[gram-positive]] [[streptococci]] surrounded by pus cells](https://commons.wikimedia.org/wiki/Special:FilePath/Gram-positive bacteria and pus cells.jpg?width=200 "Gram-stain of [[gram-positive]] [[streptococci]] surrounded by pus cells")
![Purple-stained [[gram-positive]] (left) and pink-stained [[gram-negative]] (right)](https://commons.wikimedia.org/wiki/Special:FilePath/Gram Staining Bacteria.jpg?width=200 "Purple-stained [[gram-positive]] (left) and pink-stained [[gram-negative]] (right)")
MICROBIOLOGICAL METHOD FOR IDENTIFICATION; METHOD OF STAINING USED TO DIFFERENTIATE BACTERIAL SPECIES INTO TWO LARGE GROUPS (GRAM-POSITIVE AND GRAM-NEGATIVE)
Gram staining; Gram's stain; Gram-variable; Gram status; Gram's method; Gram Stain; Gram-stain; Gram-staining; Gram-categorization; Gram-indeterminate; Gram indeterminate; Gram Staining; Gram indeterminant; Gram variable
In microbiology and bacteriology, Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884.
Gram stain
MICROBIOLOGICAL METHOD FOR IDENTIFICATION; METHOD OF STAINING USED TO DIFFERENTIATE BACTERIAL SPECIES INTO TWO LARGE GROUPS (GRAM-POSITIVE AND GRAM-NEGATIVE)
Gram staining; Gram's stain; Gram-variable; Gram status; Gram's method; Gram Stain; Gram-stain; Gram-staining; Gram-categorization; Gram-indeterminate; Gram indeterminate; Gram Staining; Gram indeterminant; Gram variable
¦ noun Medicine a staining technique for the preliminary distinction of bacteria between two categories (Gram-positive and Gram-negative).
Origin
C19: named after the Danish physician Hans C. J. Gram.
Wikipédia
DAPI
DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore provides a marker for membrane viability.
